開発年:2003年開発者:目加田英輔先生機関名:大阪大学 微生物病研究所BALB/c に対し、10世代バッククロス Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> HB-EGF Knockout mice(BALB) (HBdel#80/BALB) HB-EGF Knockout mice(BALB) (HBdel#80/BALB) <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/apr_2006_mm" target="_blank">Mouse of the Month Apr 2006</A> Developed by Drs Ryo Iwamoto, Eisuke Mekada et al., at Osaka University in 2003. The HB-EGF null mice were generated by crossing with CAG-Cre transgenic mice. 国立大学法人大阪大学 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>利用者が本件リソース寄託後、2年以内に理研BRCより提供を受けて得た研究成果の発表は寄託者(開発者)との共著とする。 TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] phage P1 loxP site, E. coli lacZ, poly A, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, poly A, mouse heparin-binding epidermal growth factor-like growth factor genomic DNA Osaka Univ. 出生後、かなりの確率で早期に致死となるため、ヘテロでの交配維持が必要。形態の異常・奇形があるホモで不妊/死亡 Eisuke MEKADA RBRC01307 開発年:2003年 開発者:目加田英輔先生 機関名:大阪大学 微生物病研究所 BALB/c に対し、10世代バッククロス <a href='https://brc.riken.jp/mus/pcr01307'>Genotyping protocol -PCR-</a> C(3〜6か月) Hbegf, heparin-binding EGF like growth factor is a member of the EGF family of growth factor. This protein binds to and activates the EGF receptor. Homozygous mutant mice exhibit grossly enlarged cardiac valves and ventricular chambers. Most homozygous mutant mice die by 3 weeks of age. LacZ gene is expressed under the native HB-EGF promoter to examine the expression pattern of the HB-EGF histologically. HB-EGF Knockout mice(BALB) (HBdel#80/BALB) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. 出生後、かなりの確率で早期に致死となるため、ヘテロでの交配維持が必要。 形態の異常・奇形がある ホモで不妊/死亡 C.Cg-Hbegf<tm1.1Mek>/MekRbrc Backcross to BALB/c (Heterozygote x BALB/cCrSlc) Most homozygous mutant mice were die by 3 weeks of age. 目加田 英輔 Developed by Drs Ryo Iwamoto, Eisuke Mekada et al., at Osaka University in 2003.The HB-EGF null mice were generated by crossing with CAG-Cre transgenic mice. C (3-6 months) 心臓拡張、機能不全、心臓弁拡張の症状を呈するため、心臓疾患のモデルマウスとなりうる。 Cardiovascular Research true Backcross to BALB/c (Heterozygote x BALB/cCrSlc)Most homozygous mutant mice were die by 3 weeks of age.